论文部分内容阅读
目的:探讨干扰素-γ(IFN-γ)及白细胞介素-10(IL-10)在子痫前期患者血清中的表达及意义。方法:选取2013年1~12月在该院产科住院的25例重度子痫前期患者(重度子痫前期组)及25例轻度子痫前期患者(轻度子痫前期组)为研究对象,另选取同期在该院住院的相应孕周正常孕妇25例为正常妊娠组。应用双抗体夹心酶联免疫吸附法(ELISA法)分别测定3组血清中IFN-γ及IL-10水平,并计算IFN-γ/IL-10比值;分析子痫前期患者血清中IFN-γ及IL-10水平与病情程度临床指标的相关性。结果:轻、重度子痫前期组血清IFN-γ水平均高于正常妊娠组,差异有统计学意义(P<0.05),而重度子痫前期组血清IFN-γ水平高于轻度子痫前期组,差异有统计学意义(P<0.05)。轻、重度子痫前期组血清IL-10水平均低于正常妊娠组,差异有统计学意义(P<0.05),而重度子痫前期组血清IFN-γ水平低于轻度子痫前期组,差异有统计学意义(P<0.05)。随着患者病情的加重,3组患者血清IFN-γ/IL-10比值逐渐增加,3组间比较差异有统计学意义(P<0.05)。子痫前期患者血清中IFN-γ及IL-10水平与子痫前期疾病严重程度具有相关性。结论:IFN-γ及IL-10的表达失衡和紊乱可能是子痫前期免疫学发病机制中的重要环节,IFN-γ与IL-10可作为其病情预测指标。
Objective: To investigate the expression and significance of interferon-γ (IFN-γ) and interleukin-10 (IL-10) in sera of patients with preeclampsia. Methods: Twenty-five patients with severe preeclampsia (severe preeclampsia) and 25 patients with mild preeclampsia (mild preeclampsia) who were hospitalized in the obstetrics department from January 2013 to December 2013 were selected as research objects. Another selection of the same period in the hospital corresponding gestational age normal pregnant women 25 cases of normal pregnancy group. Serum levels of IFN-γ and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA) and the ratio of IFN-γ / IL-10 was calculated. The levels of IFN-γ in serum of preeclampsia patients and Correlation between the level of IL-10 and clinical indicators of disease severity. Results: Serum levels of IFN-γin mild and severe preeclampsia were significantly higher than those in normal pregnancy (P <0.05), while serum levels of IFN-γ in severe preeclampsia were higher than those in mild preeclampsia Group, the difference was statistically significant (P <0.05). Serum levels of IL-10 in mild and severe preeclampsia group were lower than those in normal pregnancy group, the difference was statistically significant (P <0.05), while serum IFN-γlevel in severe preeclampsia group was lower than that in mild preeclampsia group, The difference was statistically significant (P <0.05). As the patient’s condition aggravated, the serum IFN-γ / IL-10 ratio gradually increased in the three groups, with significant difference between the three groups (P <0.05). The serum levels of IFN-γ and IL-10 in preeclampsia are correlated with the severity of preeclampsia. Conclusion: The imbalance and disorder of IFN-γ and IL-10 expression may play an important role in the immunological pathogenesis of preeclampsia. IFN-γ and IL-10 may serve as predictors of their disease status.