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目的： 补充观察我国浙江、江西、台湾省和日本山梨的日本血吸虫成虫的蛋白组分， 以及上述４ 地和安徽、湖北、四川、云南省血吸虫与广西省钉螺和日本钉螺抗血清的反应性。方法： 采用ＳＤＳ－聚丙烯酰胺凝胶电泳 （ＳＤＳ－ＰＡＧＥ） 和酶联免疫电转移印迹 （ＥＩＴＢ） 分析。结果与结论： 浙江、江西、台湾省和日本的血吸虫隔离群雄虫及雌虫抗原经ＳＤＳ－ＰＡＧＥ后， 以考马斯亮蓝染色结果分别可见７～１７ 条（江西省和日本的血吸虫雄虫条带显色很淡） 及１～６ 条显色带；银染结果分别可见１４～２３条及４～１９ 条（台湾省的血吸虫雌虫仅见１条极淡的）显色带。台湾省血吸虫雄虫与浙江省血吸虫雄虫谱型相似， 但在８１ ｋＤａ以上组分有所不同， 江西省血吸虫雄虫５４ｋＤａ 条带明显， 日本的血吸虫雄虫不仅条带少， 谱型也与浙江、江西及台湾省的血吸虫各异。ＥＩＴＢ结果： 所试血吸虫成虫与广西省钉螺和日本的钉螺抗血清均产生反应， 证明存在交叉反应抗原组分。所试血吸虫成虫与广西省钉螺和日本的钉螺间存在共同抗原组分。
OBJECTIVE: To observe the protein components of Schistosoma japonicum adults in Zhejiang, Jiangxi, Taiwan and Yamanashi of Japan, and the reactivity of schistosoma japonicum and antheraea japonica from four provinces in Anhui, Hubei, Sichuan and Yunnan Provinces. Methods: SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent blot (EITB) analysis were used. RESULTS AND CONCLUSION: Sera from Schistosoma japonicum isolates and female antigens in Zhejiang, Jiangxi, Taiwan and Japan were detected by SDS-PAGE after staining with Coomassie brilliant blue, respectively. Seven to 17 (schistosoma malaria band in Jiangxi Province and Japan Color is very light) and 1 to 6 bands; silver staining results were visible 14 to 23 and 4 to 19 (Taiwan Schistosome females see only a very light) with the color band. Schistosoma japonicum in Taiwan is similar to that of schistosoma japonicum in Zhejiang Province. However, the components of 81kDa and above are different. The 54kDa band of Schistosoma japonicum in Jiangxi Province is obvious. Schistosoma japonicum is different in Zhejiang, Jiangxi and Taiwan provinces. Results of EITB: The tested adult schistosomiasis reacted with both the snails in Guangxi and the Japanese snails antisera, demonstrating the presence of cross-reacting antigens. The adult schistosomiasis tested shared common antigenic components with the snail in Guangxi province and the snail in Japan.