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【目的】分析黄芩苷对阪崎克罗诺杆菌生物膜的抑制作用。【方法】采用XTT法评价黄芩苷对阪崎克罗诺杆菌起始粘附性及生物膜内细菌细胞活性的影响,并且采用荧光实时定量PCR(Quantitative real-time PCR,q RT-PCR)检测了阪崎克罗诺杆菌生物膜相关基因glp Q、nlp D、gsi B、deo B、lux S、sdi A的表达水平。【结果】黄芩苷对阪崎克罗诺杆菌的抑制效果呈剂量依赖型。黄芩苷对阪崎克罗诺杆菌的MIC80值为1 024 mg/L,该浓度的黄芩苷对阪崎克罗诺杆菌BAA-894和IQCC10423菌株生物膜的抑制率分别为83.7%和53.2%。浓度为2 048 mg/L的黄芩苷能够通过降低阪崎克罗诺杆菌的粘附性来抑制新生物膜的形成。另外,实时定量PCR结果表明黄芩苷可能通过下调阪崎克罗诺杆菌生物膜相关基因的表达来抑制其生物膜的形成。【结论】黄芩苷有可能被作为抗菌剂以预防和灭活阪崎克罗诺杆菌生物膜。
【Objective】 To analyze the inhibitory effect of baicalin on the biofilm of Corynebacterium sapernatus. 【Method】 The effects of baicalin on the initial adhesion of Corynebacterium sakazakii and the activity of bacterial cells in biofilm were evaluated by XTT method. Quantitative real-time PCR (q RT-PCR) The expression level of GLP Q, nlp D, gsi B, deo B, lux S, 【Result】 The results showed that the inhibitory effect of baicalin on Corynebacterium sinica was dose-dependent. The MIC80 value of baicalin against Corynebacterium ossei was 1024 mg / L. The inhibitory rates of baicalin to biofilms of Corynebacterium Sakhangis BAA-894 and IQCC10423 were 83.7% and 53.2%, respectively. Baicalin at a concentration of 2,048 mg / L can inhibit the formation of new biofilm by reducing the adhesion of Canker’s Sakonaga. In addition, real-time quantitative PCR results showed that baicalin may inhibit the formation of biofilm by down-regulating the expression of genes related to Corynebacterium Sakanakis biofilm. 【Conclusion】 Baicalin may be used as an antibacterial agent to prevent and inactivate Corynebacterium kamazaki biofilms.