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我们于1988年8月,1989年6月,1990年5月连续3年对怀来县城内的部分理发业、旅店业进行HBsAg污染情况调查。 采样时,将无菌的磷酸盐缓冲液1ml装入无菌试管内(12mm×130mm),用无菌棉签蘸PBS(pH7.4)液后涂抹检样表面。将棉签上的液体在管壁上用力挤压后,放置室温过夜。反向间接血凝试验采用卫生部北京生物制品研究所生产的HBs-Ag诊断血球并按说明上标准进行检测样品。
In August 1988, June 1989, May 1990 for three consecutive years in Huailai County, part of the hairdressing industry, hotel industry HBsAg pollution survey. Sampling, sterile sterile phosphate buffer 1ml into a sterile tube (12mm × 130mm), sterile cotton swab dipped in PBS (pH7.4) and smear the sample surface. After swabbing the liquid on the swab tightly against the wall of the tube, allow to stand at room temperature overnight. Reverse indirect hemagglutination test HBs-Ag produced by the Beijing Institute of Biological Products, Ministry of Health, was used to diagnose blood cells and samples were tested according to the standard.