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采用造血祖细胞体外培养。造血生长因子生物活性检测等实验血液学技术，研究维生素Ａ缺乏（ＶＡＤ）对大鼠红系早期祖细胞（ＢＦＵ－Ｅ）增殖调控的影响。结果发现，与对照组比较，ＶＡＤ使ＢＦＵ－Ｅ集落增殖明显减少；用ＶＡＤ的脾及骨髓基质细胞培养上清液明显抑制ＢＦＵ－Ｅ集落增殖。结果提示，ＶＡＤ可能通过抑制造血生长因子白细胞介素３（ＩＬ－３）和粒单系集落刺激因子（ＧＭ－ＣＳＦ）等表达而影响红系造血。
Hematopoietic progenitor cells cultured in vitro. Hematopoietic growth factor bioassay and other experimental hematological techniques to study the effects of vitamin A deficiency (VAD) on the proliferation of rat erythroblast precursor progenitor cells (BFU-E). The results showed that, compared with the control group, VAD significantly reduced the proliferation of BFU-E colonies; the supernatant of VAD spleen and bone marrow stromal cells significantly inhibited the proliferation of BFU-E colonies. The results suggest that VAD may affect erythroid hematopoiesis by inhibiting the expression of hematopoietic growth factor interleukin 3 (IL-3) and granulocyte colony stimulating factor (GM-CSF).