论文部分内容阅读
目的:探讨癌基因HER-2/neu及转移抑制基因nm23在喉鳞状细胞癌(LSCC)中的表达及与局部浸润和淋巴结转移的关系。方法:采用免疫组织化学SP法,检测56例LSCC患者手术切除的癌组织中HER-2/neu及nm23蛋白的表达,并以正常喉黏膜组织做对照。结果:HER-2/neu蛋白阳性表达率与喉癌组织的分化程度无明显相关性(P>0.05),与临床分期和淋巴结转移呈正相关(P<0.05或P<0.01);nm23蛋白阳性表达率无淋巴结转移者显著高于有淋巴结转移者(P<0.01),与LSCC淋巴结转移呈负相关。而且,在淋巴结转移者中HER-2/neu蛋白的阳性表达率与nm23蛋白呈负相关。结论:HER-2/neu及nm23蛋白异常表达在LSCC发生、发展和淋巴结转移中可能起重要作用。
Objective: To investigate the expression of oncogene HER-2 / neu and metastasis suppressor gene nm23 in laryngeal squamous cell carcinoma (LSCC) and its relationship with local infiltration and lymph node metastasis. Methods: Immunohistochemical SP method was used to detect the expression of HER-2 / neu and nm23 proteins in 56 resected cancerous tissues of LSCC patients. The normal laryngeal mucosa tissues were used as controls. Results: The positive expression rate of HER-2 / neu was not significantly correlated with the differentiation of laryngeal carcinoma (P> 0.05), but positively correlated with clinical stage and lymph node metastasis (P <0.05 or P <0.01) The rate of lymph node metastasis was significantly higher than those with lymph node metastasis (P <0.01), and lymph node metastasis was negatively correlated. Moreover, the positive expression rate of HER-2 / neu protein in lymph node metastasis was negatively correlated with nm23 protein. Conclusion: The abnormal expression of HER-2 / neu and nm23 proteins may play an important role in the occurrence, development and lymph node metastasis of LSCC.