许多生化技术和医学诊断方法需要高纯度的细胞亚群样品。激光流式细胞分选术是一种新的物理分离方法,它根据单细胞水平上检测到的多种物化特性进行分选,具有高纯度的特点。这一技术已影响着生物医学的诸多领域。分选原理激光流式细胞分选的主要原理是:单分散的染色细胞在高速流动状态下依次恒速通过激光激发区,产生荧光或散射光,这些光信号反映了细胞的 DNA、RNA 和体积等参数,经变 Many biochemical and medical diagnostic methods require high purity cell subpopulation samples. Laser flow cytometry is a new method of physical separation based on the detection of a variety of physicochemical properties at the single cell level for sorting with high purity. This technique has affected many areas of biomedicine. Sorting principle Laser flow cytometry The main principle is: Monodisperse stained cells in a high-speed flow state, followed by a constant speed through the laser excitation region, resulting in fluorescence or scattered light, the optical signal reflects the cell DNA, RNA and volume Other parameters, the change