论文部分内容阅读
分别以接种感染芜菁花叶病毒(TuMV)和健康对照的青菜苏州青品种(Brassica chinensis L. cv.Suzhou)和芥菜温州芥菜品种(B.juncea L. cv.Wenzhou)叶片为材料提取完整叶绿体,用胰蛋白酶消除其表面蛋白后,抽提总蛋白,经SDS-PAGE电泳和Western blot检测,发现TuMV的外壳蛋白(CP)存在于感病寄主的叶绿体中。免疫金标记电镜实验显示TuMV-CP定位在感病青菜和芥菜的细胞质和叶绿体中。对两种寄主植物叶片的叶绿素荧光动力学参数测定结果显示,青菜、芥菜的Fv/Fo、Fv/Fm、φPSII、qp值都有不同程度的降低,qN值增大。实验结果表明TuMV侵染后在寄主细胞叶绿体中积累的CP,抑制了光系统II(PS II)的光化学活性,这可能是影响寄主植物光合作用的一个重要原因。
The whole chloroplast was extracted from the leaves of Brassica chinensis L. cv. Suzhou and B. juncea L. cv. Wenzhou which were infected with TuMV and healthy control respectively. After removing the surface protein by trypsin, the total protein was extracted. The coat protein (CP) of TuMV was found in the chloroplasts of susceptible host by SDS-PAGE electrophoresis and Western blot. Immunogold labeling electron microscopy experiments showed that TuMV-CP was localized in the cytoplasm and chloroplast of susceptible vegetables and mustard. The chlorophyll fluorescence kinetics parameters of two host plant leaves showed that the Fv / Fo, Fv / Fm, φPSII and qp values of Chinese cabbage and mustard decreased with different degrees and qN values increased. The experimental results show that the accumulation of CP in the host cell chloroplast after TuMV infection inhibits the photochemical activity of PS II, which may be one of the important factors that affect the photosynthesis of host plants.