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对黑龙江、吉林、辽宁、安徽、山东、内蒙、河南、河北、江苏、安徽、浙江、湖北、福建、广东、海南、上海等省、市猪群进行了猪流感的血清学和病原学调查研究。从 130 6份猪鼻棉拭子样品和死亡猪肺、气管样品中分离到 39株H3N2亚型猪流感病毒 ,12株 H1亚型猪流感病毒及其它亚型猪流感病毒 ,病毒粒子在透射电镜下呈现典型的正粘病毒特征 ,有囊膜和纤突 ,多为椭圆形、圆形、或杆形 ,直径约 80～ 12 0 nm。对 1997- 2 0 0 1年 3895份猪血清的血清学调查结果表明 ,近年中国多个地区猪群存在抗SWTN77(H1)、SWCO77(H3)、DKAT6 9(H1)、TKUK6 3(H3)流感病毒抗体 ;1998年来自河北的猪血清中抗 SWTN77阳性率高达 33.3% ;1999年来自内蒙的猪血清中抗 TKUK6 3和SWCO77阳性率分别为 13.3%和 2 6 .7% ;2 0 0 0- 2 0 0 1年调查的所有省市的猪群中均存在抗SWCO77抗体 ,阳性率从 3.2 %～ 10 0 %。说明近年我国猪群中 H3亚型猪流感的污染相当普遍
The serological and etiological investigation of swine influenza in pigs in Heilongjiang, Jilin, Liaoning, Anhui, Shandong, Inner Mongolia, Henan, Hebei, Jiangsu, Anhui, Zhejiang, Hubei, Fujian, Guangdong, Hainan and Shanghai . Thirty-nine H3N2 subtype swine influenza virus, 12 H1 subtype swine influenza virus and other subtype swine influenza viruses were isolated from 1306 swine cotton swab swab samples and dead pig lung and tracheal samples. The virus particles were analyzed by transmission electron microscopy The typical features of orthomyxovirus, capsule and fiber process, mostly oval, round, or rod-shaped, about 80 ~ 120 nm in diameter. The results of serological investigation of 3895 swine sera from 1997 to 2001 showed that there are anti-SWTN77 (H1), SWCO77 (H3), DKAT6 9 (H1) and TKUK6 3 (H3) The antibody against SWTN77 in swine serum from Hebei Province in 1998 was as high as 33.3%. In 1999, the positive rates of anti-TKUK6 3 and SWCO77 in pig serum from Inner Mongolia were 13.3% and 26.7%, respectively. The anti-SWCO77 antibody was present in all provinces and municipalities surveyed in 2001, the positive rate was 3.2% ~ 100%. This shows that the swine flu in the H3 subtype of swine in China is very common in recent years