目的了解四川省ＨＩＶ１各亚型的流行情况。方法采集四川省内检出的１２例ＨＩＶ１感染者的全血标本，提取核酸后用套式聚合酶链反应（ＮｅｓｔｅｄＰＣＲ）技术扩增ＨＩＶ１ｅｎｖ基因区，用异源双链电泳泳动法进行ＨＩＶ１亚型的分析。结果１２份标本中有１１份ＰＣＲ扩增阳性，亚型分析证明１１份标本均为Ｂ亚型。结论ＨＩＶ１Ｂ亚型可能是四川省流行的主要亚型。 Objective To understand the prevalence of HIV-1 subtypes in Sichuan Province. Methods The whole blood samples from 12 infected HIV-1 patients in Sichuan province were collected. After nucleic acid extraction, the HIVenv gene region was amplified by NestedPCR. The heteroduplexes Electrophoresis electrophoresis swimming HIV 1 subtype analysis. Results Of the 12 samples, 11 were positive for PCR amplification. The subtype analysis showed that 11 samples were all subtype B. Conclusion HIV 1 subtype 1B may be the main subtype of epidemic in Sichuan Province.