在对着色芽生菌病致病菌种的生物学特征、菌种分类和药敏观察等方面的实验研究中，制备单孢子悬液是其重要的一环。既往所采用的吹打、研磨等方法，常不能获得满意结果。我们参考了有关文献介绍的方法［１，２］并加以改进，结果满意，兹介绍如下。１材料和方法１．１材料：采 In the study on the biological characteristics, species classification and drug susceptibility observation of pathogenic bacteria of colored mycobacteria, preparation of single spore suspension is an important part. Past used by blowing, grinding and other methods, often can not get satisfactory results. We have made reference to and improved the methods [1, 2] introduced in the literature, with satisfactory results, which are introduced below. 1 Materials and Methods 1.1 Materials: Mining