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应用Real-time RT-PCR检测了水稻条纹病毒(Rice stripe virus,RSV)在2种水稻品种(品系)武育粳3号和KT95-418的悬浮细胞内复制变化和相对含量的差异,结合传统生物学接种试验,确定了这2个品种(品系)对RSV抗性的差异.结果表明,RSV在武育粳3号的悬浮细胞内24 h达到复制高峰,病毒含量为侵染初期的7.46倍.而在KT95-418的悬浮细胞内,RSV达到复制高峰需要36 h,病毒含量为侵染初期的4.51倍.利用病毒生物学接种的方法,武育粳3号发病率达91.7%,而KT95-418仅为36.0%.由此可见,KT95-418较武育粳3号对RSV具有较高的抗病性.因此,Real-time RT-PCR方法与传统生物学接种试验方法相比,具有更高的准确性和灵敏性,可以作为传统品种抗病性鉴定的验证手段.
Real-time RT-PCR was used to detect the variation of intracellular replication and relative content of rice stripe virus (RSV) in two kinds of rice cultivars Wuyujing 3 and KT95-418, The results showed that RSV reached the peak of replication within 24 h in the suspension cells of Wuyujing 3, the virus content was 7.46 times of that in the initial stage of infection .In the suspension cells of KT95-418, RSV reached the cloning peak for 36 h, and the virus content was 4.51 times of the initial infection.Using the virus inoculation method, the incidence of Wuyujing 3 was 91.7%, while that of KT95 -418 was only 36.0% .Therefore, KT95-418 had higher resistance to RSV than that of Wuyujing 3. Therefore, compared with traditional biological inoculation test method, Real-time RT-PCR method has Higher accuracy and sensitivity can be used as a validation tool for the identification of traditional varieties of disease resistance.