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目的:探讨内脏敏感增高型功能性消化不良脾虚证动物模型的建立方法并进行模型的评价。方法:运用2%蔗糖溶液灌胃并复合小平台站立法制作内脏敏感增高型功能性消化不良脾虚证动物模型,通过观察大鼠的一般情况,运用ELISA试剂盒检测血清乳酸脱氢酶、血清D-木糖排泄率、血清胃动素的含量,对此模型进行评价。结果:与正常组相比,模型组大鼠的食少、纳呆、腹胀、毛发疏松不泽、神疲乏力等脾虚症状都较其他组重,且持久。在行为学上,碘乙酰胺复合小平台组大鼠的糖水消耗均低于对照组。在指标检测上,模型组大鼠血清D-木糖排泄率、胃动素含量均低于对照组,而乳酸值明显高于对照组(P<0.05或P<0.01)。结论:通过对模型组大鼠的一般情况和生物学指标的辨证分析,认为内脏敏感增高型功能性消化不良脾虚证动物模型制作成功,此模型达到了“病证结合”的要求。
Objective: To explore the establishment of animal models of visceral hypersensitivity dysfunction and spleen deficiency syndrome and to evaluate the model. Methods: Animal models of visceral hypersensitivity dysfunction and spleen deficiency syndrome were established by gavage with 2% sucrose solution and combined with a small platform station. By observing the general situation in rats, serum lactate dehydrogenase, serum D - xylose excretion rate, serum motilin content of this model to evaluate. Results: Compared with the normal group, rats in the model group had less symptoms of spleen deficiency, such as less food, poor appetite, abdominal distension, loose hair and weakness of the hair, and more fatigue than other groups. In behavioral, Iodoacetamide plateau group rats sugar consumption were lower than the control group. In the index test, the model group rats serum D-xylose excretion rate, motilin content were lower than the control group, while the lactic acid value was significantly higher than the control group (P <0.05 or P <0.01). Conclusion: Through the analysis of the general situation and biological indexes of model rats, it is considered that the animal model of spleen deficiency syndrome with visceral hypersensitivity dysfunction is successful. The model has reached the requirement of “combination of disease and syndrome”.