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目的:利用高效液相色谱法对天麻胶囊中天麻素的含量进行定量分析。方法:采用Kromasil C18(4.6 mm×200 mm,5μm)色谱柱,流动相为甲醇-水(55∶45),流速1.0 mL.min-1,检测波长220 nm,进样量10μL。结果表明天麻素浓度在8～80μg.mL-1范围内具有良好的线性关系(r=0.9995),天麻素平均回收率(n=6)为98.87%(RSD=1.4%)。结论:本法结果准确,操作简便,适合用于天麻胶囊的质量控制分析检验。
OBJECTIVE: To quantitatively analyze the content of gastrodin in Tianma capsule by high performance liquid chromatography. Methods: Kromasil C18 column (4.6 mm × 200 mm, 5 μm) was used. The mobile phase was methanol-water (55:45) and the flow rate was 1.0 mL.min-1. The detection wavelength was 220 nm and the injection volume was 10 μL. The results showed that gastrodin had a good linearity (r = 0.9995) in the range of 8 ~ 80μg.mL-1 and 98.87% (RSD = 1.4%) in the average recovery of gastrodin. Conclusion: The method is accurate, easy to operate and is suitable for the quality control analysis of Tianma capsule.