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目的:利用水产品中分离到的耐药大肠埃希菌,研究整合子与耐药性的关系。方法:PCR扩增1类和2类整合酶及整合子,将其进行序列分析。结果:85株耐药大肠埃希菌中88%含有1类整合酶基因;其中68%能扩增此基因;1类整合子可变区PCR扩增产物谱型有17种;1类整合子最常见的基因盒有4种。还发现1株大肠埃希菌同时携带1和2两类不同的整合酶和整合子。结论:大肠埃希菌耐药性与整合子有相关性。
Objective: To study the relationship between integron and drug resistance by using drug-resistant Escherichia coli isolated from aquatic products. Methods: Type 1 and 2 integrase and integron were amplified by PCR and sequenced. RESULTS: 88% of the 85 strains of Escherichia coli isolated from Escherichia coli contained type 1 integrase genes, of which 68% could amplify this gene. There were 17 types of PCR amplification products of type 1 integron. The most common gene cassette has 4 kinds. One strain of Escherichia coli was also found to carry two different types of integrase and integrons, one and two. Conclusion: The drug resistance of Escherichia coli is related to integron.