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建立大鼠血浆中灵芝酸单体T的高效液相色谱测定方法,并应用于其药动学研究。采用反相色谱柱,检测波长为245 nm,流动相为甲醇-水-乙酸(95.5:4.5:0.5,v/v/v);流速为1 mL/min;内标为丙酸睾酮。灵芝酸单体T血浆浓度在0.05～50μg/mL范围内线性良好(r>0.999),定量限为20ng/mL,低、中、高浓度质控样品回收率分别为97.6%,98.4%和103.2%,日内、日间精密度(以相对标准偏差表示)不超过8.2%。大鼠静脉给药主要药动学参数为:t_(1/2α):(5.46±1.25)min;t_(1/2β):(227.18±11.40)min;CL:(1.09±0.16)mL/(kg·min);AUC_(0-12h):(3939.13±311.14)μg·min/mL;AUC_(0-∞):(4681.96±710.70)μg·min/mL,绝对生物利用度为41.98%±2.40%。该方法快速简便、灵敏度高、定量准确,适用于大鼠体内灵芝酸的药物动力学研究。
To establish a method for the determination of ganoderic acid monomeric T in rat plasma by HPLC and to apply it in its pharmacokinetic study. The mobile phase was methanol-water-acetic acid (95.5: 4.5: 0.5, v / v / v). The flow rate was 1 mL / min. The internal standard was testosterone propionate. The linearity (g> 0.999) for ganoderic acid monomeric T plasma was linear over the range of 0.05-50 μg / mL with a limit of quantification of 20 ng / mL. The recoveries of low-, middle- and high-concentration QC samples were 97.6%, 98.4% and 103.2, respectively %, Day, day precision (relative standard deviation) does not exceed 8.2%. The main pharmacokinetic parameters of intravenous administration in rats were as follows: t 1/2 (5.46 ± 1.25) min; t 1/2 / (227.18 ± 11.40) min; CL 1.09 ± 0.16 mL / ( kg · min); AUC_ (0-12h) :( 3939.13 ± 311.14) μg · min / mL; AUC_ (0-∞) :( 4681.96 ± 710.70) μg · min / mL and absolute bioavailability was 41.98% ± 2.40 %. The method is rapid, simple, sensitive and quantitative. It is suitable for the pharmacokinetic study of Ganoderma lucidum in rats.