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以莲叶海棠幼叶为材料，材料经表面消毒接种于诱导培养基上，２７ｄ左右外植体表面出现不定芽．经继代培养可获大量不定芽．不定芽在壮苗培养基上培养４５ｄ转至生根培养基中，４０ｄ后长出完整根系．再生植株炼苗后转移至砂基中进行培养，移栽成活率为９０％．
The leaves of Begonia lotus were used as material, and the materials were inoculated on the induction medium by surface disinfection. Adventitious buds appeared on the surface of explants on the 27th day. After subculture can be a large number of adventitious buds. Adventitious buds were cultured on strong seedlings for 45 days and then transferred to rooting medium. After 40 days, whole roots were grown. Regenerated plants were harvested and transferred to sand-based medium for culture. The survival rate of transplanting was 90%.