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目的:观察豆根管食通口服液对人食管癌109细胞多药耐药基因糖蛋白P-170表达的影响。方法:通过实验分组,用MTT法(四甲基氮唑蓝法)测定药物对肿瘤细胞增殖的抑制作用,用免疫组化法测定药物对肿瘤细胞P-170的作用。结果:单用豆根管食通100,150,200 mg.mL-1组A值明显低于单用氟尿嘧啶(5-Fu)组,其他组差别不大;豆根管食通口服液200mg.mL-1组及豆根管食通口服液100 mg.mL-1联合25μg.mL-15-Fu组P-170表达均较其他低浓度组低。结论:豆根管食通口服液对人食管癌109细胞增殖有明显的抑制作用,与药物浓度呈正相关;高浓度豆根管食通口服液可明显降低人食管癌细胞P-170的表达。
OBJECTIVE: To observe the effect of Douge Guanteng oral liquid on the expression of multidrug resistance gene glycoprotein P-170 in human esophageal carcinoma 109 cells. Methods: The inhibitory effects of drugs on the proliferation of tumor cells were determined by MTT assay (MTT method). The effect of drugs on P-170 tumor cells was determined by immunohistochemistry. Results: The A value of 100,150,200 mg.mL-1 group was significantly lower than that of 5-Fu alone group, while the other groups were not different. 200 mg.mL-1 group of Beansutangxutong oral liquid The expression of P-170 in 100 mg.mL-1 combined with 25 μg.mL-15-Fu group was lower than that in other low-concentration groups. CONCLUSION: Doudengshuantong Oral Liquid can significantly inhibit the proliferation of human esophageal carcinoma 109 cells and has a positive correlation with the drug concentration. High concentration of Dougan Guanhuitong oral liquid can significantly reduce the expression of P-170 in human esophageal cancer cells.