作者制备抗原的方法是在枭猴感染恶性疟原虫或间日疟原虫后裂殖体原虫血症达高峰时,用含有1,000单位肝素的针筒从心脏或肺静脉抽取猴血,在4℃内以1,100g离心10分钟,除去血浆,将细胞混悬于相当于原血量的冷的(4～6℃)生理盐水中,再离心,弃去盐水和血块黄色层后,继续洗2次,将细胞混 The authors prepared antigens by collecting blood from the heart or pulmonary vein of a syringe containing 1,000 units of heparin at the peak of schistosome parahaemolyticus after the infection of P. falciparum or P. vivax After centrifugation at 1,100g for 10 minutes, the plasma was removed, and the cells were suspended in cold (4-6 ° C) physiological saline corresponding to the original amount of blood. After centrifugation and discarding the saline and clotted yellow layer, Mixed cells