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背景:乌龙丹是在长期临床用于缺血性脑血管病治疗中总结出来的经验方,该方以“益气健脾补肾,活血通络化痰”立法。以前的研究表明该方具有改善大脑微循环,调节神经内分泌和抗脑缺血损伤等作用。血液黏度升高,血管及神经细胞等分泌的内皮素、一氧化氮(NO)失调参与了多梗死性痴呆(multi-infarctdementia,MID)病理发展过程,且相互影响。目的:通过复制MID大鼠模型,探讨乌龙丹对MID模型大鼠血液黏度、NO及内皮素的影响。设计:以实验动物为研究对象的随机对照实验研究。单位:一所军医大学的中医系。材料:实验于2002-01/08在解放军第一军医大学中医系实验室和附属珠江医院神经内科实验室完成。选用清洁级SD大鼠雄性大鼠,体质量(270±30)g(合格证号2000337)。实验分为空白对照组、模型组、乌龙丹低剂量组、乌龙丹高剂量组。方法:在分离一侧颈内动脉后,推注1∶200比例的同种属的大鼠干血悬液0.8mL制作MID大鼠模型。血液黏度测定采用锥板法,内皮素测定采用放射免疫法,NO测定采用镉还原比色法,乌龙丹的制剂采用水煮醇提法。主要观察指标:主要指标:各实验组全血黏度和血浆黏度、血浆内皮素、NO浓度。次要指标:学习记忆测试结果。结果:模型组大鼠全血黏度(mPa·s)显著升高(200s-1:7.21±1.02;5s-1:11.24±0.93,P<0.01
BACKGROUND: Wulongdan is a long-term clinical experience used in the treatment of ischemic cerebrovascular disease. The party is enshrined in the policy of “invigorating qi, strengthening the spleen and tonifying the kidney, and promoting blood circulation to clear the blood and resolving phlegm.” Previous studies have shown that this side can improve cerebral microcirculation, regulate neuroendocrine and anti-cerebral ischemic injury. Increased blood viscosity, imbalance of endothelin and nitric oxide (NO) secreted by blood vessels and nerve cells are involved in the pathogenesis of multi-infarct dementia (MID) and interact with each other. OBJECTIVE: To investigate the effects of Wulongdan on blood viscosity, NO, and endothelin in MID rat models by replicating the MID rat model. Design: A randomized, controlled experimental study of experimental animals. Unit: Department of Traditional Chinese Medicine, a military medical university. MATERIALS: The experiment was performed at the Laboratory of Traditional Chinese Medicine, First Military Medical University of Chinese PLA and the Laboratory of Neurology, Zhujiang Hospital Affiliated to the PLA from January 2002 to August 2008. Clean SD rat male rats were used and the body weight was (270±30) g (QC No. 2000337). The experiments were divided into blank control group, model group, Wulongdan low dose group, and Wulongdan high dose group. METHODS: After one side of internal carotid artery was dissected, 0.8 mL of dry blood suspension of rat of the same species was injected in a ratio of 1:200 to make a MID rat model. Blood viscosity was measured by cone-plate method, endothelin was measured by radioimmunoassay, NO was determined by cadmium reduction colorimetric method, and Wulongdan’s formulation was boiled with alcohol. MAIN OUTCOME MEASURES: Main indicators: Whole blood viscosity and plasma viscosity, plasma endothelin, and NO concentration in each experimental group. Secondary indicators: learning memory test results. RESULTS: The blood viscosity (mPa·s) of the model group increased significantly (200s-1:7.21±1.02; 5s-1:11.24±0.93, P<0.01).