甲氧西林（methicillin）是第一个半合成的青霉素，原有的测定方法都比较复杂。作者根据甲氧西林结构中第6位含有一个激发后可发出荧光的基团，建立了一种测定甲氧西林的快速荧光法。用pH6．20的缓冲液，可测定0．03～10．0yg·mL’浓度的甲氧西林；检定和测定限度分别为0．018 Methicillin (methicillin) is the first semi-synthetic penicillin, the original determination of the methods are more complicated. The authors established a rapid fluorimetric assay for the determination of methicillin based on the inclusion of an excited fluorescence group at position 6 in the methicillin structure. With pH6.20 buffer, measurable concentration of 0.03 ~ 10.0yg · mL methicillin; test and determination limits were 0.018