龙胆是一种常用的中草药,花朵绚丽,具有很高的观赏价值,我们对它进行了快速繁殖和试管苗开花的研究,均已获得成功。简介方法如下: 一、试管苗的培养以条叶龙胆(Gentiana manshurica Kitag.)种子胚为材料,按一般组织培养操作程序,消毒灭菌,接种在含有0.5毫克/升吲哚乙酸,0.2毫克/升玉米素,0.05毫克/升生物素的MS培养 Gentiana is a commonly used Chinese herbal medicine, with brilliant flowers and high ornamental value. We have succeeded in researching its rapid propagation and in vitro plantlet flowering. Introduction methods are as follows: First, the culture of test tube seedlings Gentiana manshurica Kitag seed embryo as a material, according to the general tissue culture operating procedures, disinfection and inoculation in 0.5 mg / l indole acetic acid, 0.2 mg / L zeatin, 0.05 mg / L biotin in MS culture