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为探讨鼻腔接种伤寒杆菌Fe SOD对鼠伤寒杆菌攻击小鼠的交叉免疫保护作用 ,用IL 1作为佐剂 ,将伤寒杆菌Fe SOD经鼻腔接种小鼠 ,再以不同剂量的鼠伤寒杆菌攻击 ,观察小鼠的存活情况。当用IL 1作为佐剂时 ,经鼻腔接种伤寒杆菌Fe SOD的小鼠在 2LD50 鼠伤寒杆菌攻击后 ,3天和 7天的存活率均明显高于对照组 (P <0 0 1或P <0 0 5 ) ,当以 5LD50 鼠伤寒杆菌攻击时 ,小鼠 7天的存活率明显高于对照组 (P <0 0 1)。结果说明伤寒杆菌Fe SOD经鼻腔接种后对鼠伤寒杆菌的攻击可产生一定的免疫保护作用 ,也进一步说明了Fe SOD是沙门氏菌的共同保护性抗原。
In order to investigate the cross-immunoprotective effect of Fe SOD against Salmonella typhimurium-challenged mice inoculated with nasal inoculation, IL 1 was used as an adjuvant to infect mice with Salmonella typhi Fe SOD via nasal cavity and then challenged with different doses of Salmonella typhimurium Mouse survival. When IL-1 was used as an adjuvant, mice survived 3 and 7 days post-challenge with Salmonella typhimurium 2LD50 inoculated intranasally with Fe SOD, which was significantly higher than that of the control group (P <0.01 or P < When challenged with 5LD50 Salmonella typhimurium, the 7-day survival rate of mice was significantly higher than that of the control group (P <0.01). The results showed that Salmonella typhimurium Fe SOD after Salmonella typhimurium challenge can produce some immune protection, but also further demonstrates that Fe SOD is a common protective antigen of Salmonella.