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土壤微生物是土壤生态功能的重要指示物,因此,研究其群落特征对指导煤矸山复垦具有重要意义。文中以山西省曹村煤矿复垦3a(R-3a)、5a(R-5a)和7a(R-7a)煤矸山为例,用磷脂脂肪酸法(PLFA)测定了3种复垦年限土壤微生物总浓度和群落浓度,分析了对土壤质量变化的敏感菌种。结果表明:1)复垦煤矸山土壤微生物总浓度和群落浓度随年限增加均有不同程度增加,表明煤矸山复垦土壤质量状况总体有改善。2)3种复垦样地微生物群落结构的差异及其总浓度和群落浓度低于普通果园(CK)的事实表明复垦地土壤质量仍难达到与CK相当的状况。3)普通细菌中i15:1 G和16:0、真菌中18:1 w9c、革兰氏阳性菌中i15:0和a15:0、阴性菌中11Me18:1 w7c和18:1 w5c可指示复垦土壤质量的改善。
Soil microorganism is an important indicator of soil ecological function. Therefore, studying its community characteristics is of great significance to guide the reclamation of coal gangue. Taking the reclamation 3a (R-3a), 5a (R-5a) and 7a (R-7a) coal gangue mines in Caocun Coal Mine of Shanxi Province as an example, the contents of three soil reclamation years The total microbial concentration and the community concentration, and analyzed the susceptible species to soil quality change. The results showed that: 1) The total soil microbial concentration and community concentration in reclamation gangue increased with increasing years, which indicated that overall quality of reclaimed soil in coal gangue improved. 2) The differences in microbial community structure and the total concentration and the community concentration of the three kinds of reclamation plots were lower than that of common orchards (CK), indicating that the quality of reclaimed soil is still hard to reach the CK equivalent. 3) i15: 1 G and 16: 0 in common bacteria, 18: 1 w9c in fungi, i15: 0 and a15: 0 in Gram-positive bacteria, 11Me18: 1 w7c and 18: 1 w5c in gram- Reclamation of soil quality improvement.