The hyporheic zone plays an important role in groundwater and stream water quality protection. To investigate the stream-groundwater interaction mechanisms in the lateral hyporheic zone, this study examined Ma’an Creek in Chongqing during the dry season from December 2015 to April 2016. The water level, water temperature, pH and Cl. concentration in the hyporheic zone and groundwater were monitored in situ. The sediment permeability coefficient, stable isotopes of hydrogen and oxygen and concentration of DOC were analyzed. The results show that the water level changes of hyporheic zone and the movement of hyporheic flow were influenced significantly by the permeability coefficient of sediment. The hyporheic flow approximately10 cm from the stream bank was clearly affected by precipitation infiltration and evapotranspiration. During the study period,the groundwater recharged the stream, and the impact of groundwater on the hyporheic flow gradually decreased with the flow path. The hyporheic flow approximately 30 cm from the stream bank was still mainly affected by groundwater. Approximately10–30 cm from the stream bank, the mixing of groundwater with precipitation and stream water intensified. Due to the sediment properties, moisture accumulated approximately 10 cm from the stream bank and drained into the stream via hyporheic flow, with potential impacts on stream water quality. The investigate of stream-groundwater interaction mechanisms in the lateral hyporheic zone, this study examined Ma’an Creek in Chongqing during the dry season from December 2015 to April 2016. The water level, water temperature, pH and Cl. concentration in the hyporheic zone and groundwater were monitored in situ. The sediment permeability coefficient, stable isotopes of hydrogen and oxygen and concentration of DOC were analyzed. The results show that the water level changes of hyporheic zone and the movement of hyporheic flow were influenced significantly by the permeability coefficient of sediment. The hyporheic flow approximately 10 cm from the stream bank was clearly affected by precipitation infiltration and evapotranspiration. During the study period, the groundwater recharged the stream, and the impact of groundwater on the hyporheic flow gradually decreased with the flow path. The hypor Approximately 10-30 cm from the stream bank, the mixing of groundwater with precipitation and stream water intensified. Due to the sediment properties, bank and drained into the stream via hyporheic flow, with potential impacts on stream water quality.