论文部分内容阅读
目的 :胆汁预处理测定癌胚抗原 (CEA)并探讨胆囊胆汁CEA放射免疫分析的临床意义。方法 :取胆囊胆汁用无水乙醇处理 ,3 8± 2℃水浴吹干 ,1g/LBSA 40倍稀释 (A B法 ) ,去除胆汁中抑制物对CEA测定的干扰 ,后用常规放免法测定CEA ,同时分组观察结直肠良恶性疾病时CEA浓度的变化。结果 :重复实验和回收实验结果表明 ,此法重复 5~ 6次预处理胆汁样品后 ,变异系数 7 3 %~ 11 8% ,回收率为 5 6 7%~ 91 3 % ,比不经处理胆汁样品直接加样测定 ,检出值高出 4~ 19倍。对临床结直肠良恶性疾病和结直肠肿瘤早期隐匿性肝转移具有较高的诊断价值。结论 :胆囊胆汁CEA测定A B法预处理时间短 ,方法简便 ,是一般实验室都能开展的一种实用可靠的检测方法
Objective: To determine the clinical value of bile pretreatment for carcinoembryonic antigen (CEA) and to investigate the radioimmunoassay of gallbladder bile CEA. METHODS: Gallbladder bile was treated with absolute ethanol, dried in a water bath at 38±2°C, and diluted 1 g/LBSA 40 times (AB method). The interference of bile inhibitors on CEA was removed. CEA was measured by routine radioimmunoassay. At the same time, the changes of CEA concentration in the benign and malignant colorectal diseases were observed. RESULTS: The results of repeated experiments and recovery experiments showed that after this method was repeated 5 to 6 times, the coefficient of variation was 73% to 118% after pretreatment of bile samples, and the recovery rate was 56.7% to 91.0%, which was lower than that of untreated bile. The sample was directly added to the sample and the detected value was 4 to 19 times higher. For clinical colorectal benign and malignant diseases and colorectal cancer early occult liver metastasis has a high diagnostic value. Conclusion: The short-term pretreatment time of AB method in CEA determination of gallbladder bile is simple and convenient. It is a practical and reliable detection method that can be carried out in general laboratory.