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罐头瓶栽培黑木耳,由于瓶口大,接种易污染.为此,我们采取了薄膜封口打洞接种法,使接种速度成倍提高,杂菌感染极少。具体做法是:先将装满料的罐头瓶用12厘米见方的双层塑料薄膜和橡皮圈封口,再在薄膜中间用直径1.5厘米的锥形木棒打一接种孔,用医用胶布封严后,常压灭菌8～10小时。在无菌条件下揭开胶布
Canned bottle cultivation black fungus, due to large bottle, easy to inoculation pollution.To this end, we have adopted a film sealing hole vaccination method, the vaccination rate increased exponentially, with minimal bacterial infections. The specific approach is: first filled canned material bottles with 12 cm square double plastic film and rubber band sealing, and then in the middle of the film with a diameter of 1.5 cm tapered sticks to play a vaccination hole sealed with medical tape , Sterilization at atmospheric pressure for 8 to 10 hours. Unclog the tape under aseptic conditions