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目的:探讨SNX10过表达对人结直肠癌细胞增殖和EGFR表达的影响。方法:应用基因转染方法将SNX10质粒导入结直肠癌细胞LOVO细胞中,蛋白质印迹法方法鉴定转染后SNX10在细胞中的表达,利用四甲基偶氮唑盐(MTT)法检测SNX10对结直肠癌细胞增殖的影响,利用蛋白质印迹法和免疫荧光法观察EGFR表达量和定位情况。结果:蛋白质印迹法结果显示,SNX10质粒成功导入LOVO细胞中,SNX10的过表达使结直肠癌细胞增殖能力明显降低(F=16.76,P<0.01),EGF刺激后,过表达SNX10的细胞EGFR表达明显低于对照组,荧光定位显示SNX10和EGFR存在共定位关系。结论:SNX10通过调控EGFR表达使结直肠癌细胞增殖能力明显下降,SNX10可能是结直肠癌的增殖抑制基因。
Objective: To investigate the effect of SNX10 overexpression on proliferation and EGFR expression in human colorectal cancer cells. Methods: SNX10 plasmid was transfected into colorectal cancer LOVO cells by gene transfection method. The expression of SNX10 in transfected cells was identified by Western blotting. The expression of SNX10 was detected by MTT assay Rectal cancer cells proliferation, the use of Western blotting and immunofluorescence method to observe the expression of EGFR and localization. Results: Western blotting showed that the SNX10 plasmid was successfully transfected into LOVO cells. The overexpression of SNX10 significantly decreased the proliferation of colorectal cancer cells (F = 16.76, P <0.01). After EGF stimulation, Which was significantly lower than that of the control group. Fluorescence localization showed the co-localization of SNX10 and EGFR. Conclusion: SNX10 can significantly decrease the proliferation of colorectal cancer cells through regulating the expression of EGFR. SNX10 may be the gene of proliferation inhibition in colorectal cancer.