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1型人免疫缺陷症病毒(HIV-1)env基因编码糖基化蛋白前体,其在细胞中被蛋白酶水解成精蛋白(gp)120及gp41.已在gp41氨基末端发现高度免疫显性表位,后者能使健康的血清阳性个体产生体液免疫,在体外能特异性地抑制淋巴细胞增生.此区与Ⅰ型和Ⅱ型人嗜T淋巴细胞病毒的gp21E跨膜蛋白和多种逆转录病毒的免疫抑制(IS)区具有同源序列.作者通过基因重组获得了表达HIV-1SF2株包膜基因的重组金丝雀痘(CP)病毒和禽痘(FP)病毒,并将它的表达情况在鸡胚成纤维细胞(CEF)、猴肾细胞(Vero)及
The human immunodeficiency virus type 1 (HIV-1) env gene encodes a glycosylated protein precursor that is proteolytically hydrolyzed in cells into protamine (gp) 120 and gp41. A highly immunodominant epitope has been found at the amino terminus of gp41 Bit, which enables healthy seropositive individuals to produce humoral immunity and specifically inhibits lymphocyte proliferation in vitro.This region is associated with the gp21E transmembrane proteins of type I and type II human T-lymphotropic viruses and multiple reverse transcriptases The immunosuppressive (IS) region of the virus has homologous sequences.The recombinant canola virus (CP) virus and fowlpox virus (FP) expressing the envelope gene of HIV-1SF2 strain were obtained by gene recombination, and its expression In chick embryo fibroblasts (CEFs), monkey kidney cells (Vero) and