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建立了碘的凝胶色谱测定方法。色谱条件为：Ｓｈｉｍ－ｐａｃｋ ＤＩＯＬ－１５０柱；流动相甲醇－０．０１ｍｏｌ／Ｌ Ｈ３ＰＯ４（１０：９０）；流速为１．２ｍＬ／ｍｉｎ；柱温为３５℃；检测波长为２２４ｎｍ；线性范围是０．０１０～１．０ｍｇ／Ｌ；相关系数为０．９９９３；检测限为０．００１ｍｇ／Ｌ；相对标准偏差为１．２％～４．６％；回收率为９１％～９８％。所建立的方法已用于食盐、尿、海带等样品的测试，均取得较好的效果。
The iodine gel chromatography was established. The chromatographic conditions were: Shim-pack DIOL-150 column; mobile phase methanol-0.01 mol / L H3PO4 (10:90); flow rate of 1.2 mL / min; column temperature of 35 ° C .; detection wavelength of 224 nm; 0.010 ~ 1.0mg / L; the correlation coefficient was 0.9993; the detection limit was 0.001mg / L; the relative standard deviation was 1.2% ~ 4.6%; the recovery was 91% ~ 98%. The established method has been used for salt, urine, kelp and other samples of the test, have achieved good results.