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目的探讨血管紧张素-(1-7)[Ang-(1-7)]对血管紧张素Ⅱ(AngⅡ)诱导大鼠系膜细胞(GMC)增殖的影响。方法在AngⅡ诱导培养的GMC中,应用Ang-(1-7),通过[3H]-Thymidine及[3H]-Leucine掺入分别测定GMC的DNA、蛋白质合成;结晶紫染色检测细胞数目,观察系膜细胞增殖情况;分别用特异性AngⅡ受体1(AT1受体)拮抗剂[Sar1,Ile8]-AngⅡ和血管紧张素II受体2(AT2受体)拮抗剂PD123319与Ang-(1-7)共同培养,探讨Ang-(1-7)是否通过AngⅡ受体发挥作用。结果Ang-(1-7)呈剂量依赖性抑制AngⅡ诱导GMC的DNA、蛋白质合成及细胞数目增加;[Sar1,Ile8]-AngⅡ和PD123319不影响Ang-(1-7)的上述作用。结论Ang-(1-7)能抑制基础和AngⅡ诱导的GMC增殖,其作用的发挥不通过AngⅡ的AT1和AT2受体介导。
Objective To investigate the effects of angiotensin- (1-7) [Ang- (1-7)] on the proliferation of rat mesangial cells (GMCs) induced by angiotensin Ⅱ (AngⅡ). Methods Ang- (1-7) was induced in AngⅡ-induced GMCs and the DNA and protein synthesis of GMC was determined by [3H] -Thymidine and [3H] -Leucine incorporation respectively. The number of cells was detected by crystal violet staining. (Sar1, Ile8] -AngⅡ and angiotensin II receptor 2 (AT2 receptor) antagonist PD123319 and Ang- (1-7 ) To investigate whether Ang- (1-7) exerts its effect via the Ang II receptor. Results Ang- (1-7) inhibited DNA, protein synthesis and cell number of GMC induced by AngⅡ in a dose-dependent manner. [Sar1, Ile8] -AngⅡ and PD123319 did not affect the above effects of Ang- (1-7). Conclusions Ang- (1-7) can inhibit the proliferation of GMC induced by AngⅡ and AngⅡ, but its effect can not be mediated by AngⅡ AT1 and AT2 receptors.