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用乙酸纤维将ＧＬ－７－ＡＣＡ酰化酶基因工程菌大肠埃希氏菌Ａ５６／ｐＭＲＣＵ－３３４菌株固定化，酶反应的最适温度为５０℃，最适ｐＨ为８．０，与游离细胞一致；固定化细胞对温度和ｐＨ的稳定性比游离细胞提高。用薄板层析分析固定化细胞裂解ＧＬ－７－ＡＣＡ反应液中产物７－ＡＣＡ的含量以及ＧＬ－７－ＡＣＡ的残存量，在ｐＨ８．０．３７℃时ＧＬ－７－ＡＣＡ的转化率达９０％，产生７－ＡＣＡ的能力为１２．３４ｍｇ／（ｇ固定化细胞·ｈ）。使用２０批之后，ＧＬ－７－ＡＣＡ的转化率仍达６６％。
The GL-7-ACA acylase genetically engineered Escherichia coli A56 / pMRCU-334 strain was immobilized with acetate fiber. The optimal temperature for the enzyme reaction was 50 ° C, the optimum pH was 8.0, and the free cell The stability of the immobilized cells to temperature and pH is higher than that of free cells. The content of product 7-ACA and the residual amount of GL-7-ACA in immobilized cell lysate GL-7-ACA reaction solution were analyzed by thin layer chromatography. The conversion rate of GL-7-ACA at pH 8.0.37 ° C 90%, and the ability to produce 7-ACA was 12.34 mg / (g of immobilized cells · h). With 20 batches, the conversion of GL-7-ACA still reached 66%.