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据疫情报告,陕西省1979—1980年共发生“脊灰”患者118例。为了确诊病例和了解陕西省的主要流行型别,我们采集了63例患者的粪便进行病毒分离,其中27例同时测定了双份血清中和抗体,现总结如下。材料与方法检查对象为临床诊断为“脊灰”的患者。试验使用原代人胚肾细胞,免疫血清为北京生研所供给。每例患者一般在发病40天内连续采取粪便2—3份,接种细胞盲传两代。病毒鉴定与中和抗体测定都是采用小量中和试验法。患者双份血清系同时测定三型中和抗体,病毒抗原(为减毒疫苗株)稀释成100TCD_(50)/0.1毫升使用。
According to the report of the epidemic, there were 118 cases of polio in Shaanxi Province from 1979 to 1980. In order to confirm the diagnosis and understand the main epidemic types in Shaanxi Province, we collected the feces of 63 patients for virus isolation. Among them, 27 cases of simultaneous determination of two serum neutralizing antibodies are summarized as follows. Materials and Methods The subjects were clinically diagnosed as “polio” patients. Experimental use of primary human embryonic kidney cells, immune serum supply Beijing Institute of Health. Each patient usually takes 2-3 parts of faeces continuously in the 40 days of onset, and blindly inoculates the cells for two generations. Virus identification and neutralizing antibodies are measured using a small amount of neutralization test. Patients with double serum simultaneous determination of three types of neutralizing antibodies, viral antigens (attenuated vaccine strain) diluted to 100TCD_ (50) /0.1 ml use.