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从 2 8种随机引物中筛选出 2 5种对腐霉属的 10株计 8种腐霉进行 RAPD分析 ,发现腐霉属的DNA多态性是非常丰富的和普遍存在的 ,初步计算了 10株计 8种腐霉的 Nei氏遗传距离 ,利用类平均法得到 UPGMA系统树 ,并对它们的亲缘关系从 DNA分子水平上进行了初步分析 .结果表明 :在这 8种腐霉中 ,P. kunmingense与 P. irregulare亲缘关系最近 ;P. aphanidermatum与 P. tardicrescens的亲缘关系最近 ;P.ultimum与 P.debaryanum的亲缘关系最近 ;P.debaryanum与 P.dissotocum的亲缘关系最近 .UPGMA的系统树表明这 8种腐霉明显分成三大类 ,P.kunmingense,P.irregulare与 P.spinosum聚成一类 ;P. aphanidermatum与 P. tardicrescens聚成一类 ;P. ultimum,P.debaryanum与P.dissotocum聚成一类 .该结果与形态分类基本相符合
RAPD analysis of twenty-five 25 species of Pythium for 10 strains of Pythium was carried out by random amplified polymorphic DNA (RAPD). It was found that the DNA polymorphism of Pythium was very rich and ubiquitous. The preliminary calculation of 10 Nei ’s genetic distance of 8 kinds of Pythium strains was calculated, and the phylogenetic tree of UPGMA was obtained by class average method, and their genetic relationship was preliminary analyzed from the DNA molecular level.The results showed that among these 8 Pythium species, kunmingense has the closest genetic relationship with P. irregulare, P. aphanidermatum has the closest genetic relationship with P. tardicrescens, P.ultimum has the closest genetic relationship with P.debaryanum, and P.debaryanum has the closest relationship with P. dissotocum.The phylogenetic tree of UPGMA These eight kinds of Pythium were apparently divided into three categories, P. kunmingense, P. irregulare and P. spinosum clustered into one category; P. aphanidermatum and P. tardicrescens clustered into one category; P. ultimum, P. debaryanum and P. dissotocum The results are consistent with the morphological classification