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自从1970年 Szender等首次报导 1例暴露于聚氯乙烯尘引起的尘肺病例之后,聚氯乙烯尘是否能引起尘肺至今尚有争议。聚氯乙烯尘是否能致肺纤维化是确定聚氯乙烯能否致尘肺的关键问题。粉尘致肺纤维化是由于粉尘对肺巨噬细胞的毒性作用所致。为搞清聚氯乙烯尘是否致纤维化,我们将聚氯乙烯尘对豚鼠肺巨噬细胞的毒性作用进行了实验性观察。1 材料和方法1.1 粉尘来源和规格 作为阳性对照的石英尘从中国预防医学科学院劳研所购得的标准石英粉尘。聚氯乙烯粉尘取自天津大沽化工厂产品。粉尘在冰冻条件下在玛瑙乳钵中研磨破碎10微米以下约占50％。以上粉尘用生理盐水配制成浓度为 50 mg/ml混悬液。1.2 肺泡巨噬细胞培养 采用400g左右豚鼠按
Since the first case reported by Szender et al in 1970 of a case of pneumoconiosis caused by exposure to polyvinyl chloride dust, it is still controversial whether polyvinyl chloride dust can cause pneumoconiosis. Polyvinyl chloride dust can lead to pulmonary fibrosis is to determine whether the PVC can cause pneumoconiosis the key issues. Dust-induced pulmonary fibrosis is caused by the toxic effects of dust on lung macrophages. In order to find out whether the polyvinyl chloride dust caused fibrosis, we will PVC dust on the toxicity of guinea pig lung macrophages were observed experimentally. 1 Materials and methods 1.1 Dust sources and specifications As a positive control of quartz dust from the Chinese Academy of Preventive Medicine Institute of Labor purchased standard quartz dust. PVC dust from Tianjin Dagu chemical plant products. The dust is ground in an agate mortar under freezing conditions for about 50% below 10 microns. The above dust with saline formulated as a concentration of 50 mg / ml suspension. 1.2 alveolar macrophage cell culture using about 400g guinea pig press