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本研究收集胎龄5～41周的人胚胎53例,取胰腺,Bouin液固定,按常规制石蜡切片。部分切片经阿利新蓝-焰红、硝酸银-醛复红-铅苏木精等方法染色,部分切片作免疫组织化学PAP反应,观察胰岛A、B和D细胞。取不同胎龄的胰尾组织14例,戊二醛固定,Epon812包埋,透射电镜观察。本文的结果表明,胰腺内分泌部主要是由胰管2级以下分支的管壁上皮细胞,向周围的间充质内增生和分化而成。胎龄12周时,即可在光镜下辨认A、B及D细胞;电镜下观察,胰腺外分泌细胞在胎龄14周以后,才能见到分泌颗粒,证明胰岛细胞的分化早于外分泌细胞。测量16～36周胎儿胰腺内胰岛所占的比例,证明胚胎期胰腺内分泌部所占比例远高于成年人。本实验为临床使用人胎胰岛移植提供了参考资料。
In this study, 53 cases of human embryos with gestational age from 5 to 41 weeks were collected, fixed with Panin and fixed with Bouin, and paraffin sections were made according to the conventional method. Some sections were stained by alitake blue - flame red, silver nitrate - aldehyde complex red - lead hematoxylin and other methods, part of the sections for immunohistochemical PAP reaction observed islets A, B and D cells. 14 cases of pancreatic tail of different gestational age, glutaraldehyde fixation, Epon812 embedding, transmission electron microscopy. The results of this paper show that the pancreatic endocrine mainly by the pancreatic duct below the level 2 branch wall epithelial cells to the surrounding mesenchymal hyperplasia and differentiation. A, B and D cells could be identified under light microscope at 12 weeks of gestational age. Seen by electron microscopy, exocrine pancreatic cells could be seen after 14 weeks of gestational age, demonstrating that islet cells differentiated earlier than exocrine cells. The proportion of pancreatic islets in the fetal pancreas at 16-36 weeks was measured to show that the proportion of pancreatic endocrine cells in the embryo was much higher than in adults. This experiment provides a reference for the clinical use of human fetal islet transplantation.