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为了探讨PCR-测序法在宫颈脱落细胞样品中人乳头瘤病毒(Human papillomavirus,HPV)临床检测中的应用价值,采用HPV通用引物PGMY09/11针对HPV L1区基因序列进行PCR扩增,并通过DNA测序法对HPV进行基因分型。对于混合感染样品,利用HPV型别特异性引物PCR的方法进行基因分型。325例临床样品中,228例为HPV阳性,其中66例为混合感染。共发现27种不同的HPV型别,其中HPV 16比例最多,其次是HPV 58和52。高危型HPV检出率随病变程度加重显著性增加(P<0.05),但混合感染的比例呈下降趋势(P<0.05)。在21~30岁年龄组中,HPV感染率最高。PCR-测序法与HC2对高危型HPV检测的符合度较好(kappa=0.675)。PCR-测序法可有效地对宫颈脱落细胞样品进行HPV检测和基因分型,对大规模的HPV筛查具有潜在的应用价值。
To investigate the value of PCR-sequencing in the clinical detection of human papillomavirus (HPV) in cervical exfoliated cell samples, the HPV L1 gene sequence was amplified by PCR using HPV universal primer PGMY09 / 11 and amplified by DNA Sequencing HPV genotyping. For mixed infection samples, genotyping was performed using the HPV type-specific primer PCR method. Of 325 clinical samples, 228 were HPV positive, of which 66 were mixed infections. A total of 27 different types of HPV were found, with HPV 16 being the most common, followed by HPV 58 and 52. The detection rate of high-risk HPV increased with the severity of the disease increased significantly (P <0.05), but the proportion of mixed infections showed a downward trend (P <0.05). In the 21 to 30 age group, the highest HPV infection rate. The coincidence of PCR-sequencing and HC2 for high-risk HPV testing was good (kappa = 0.675). PCR-sequencing method can effectively detect and genotype HPV in cervical exfoliated cell samples, which has potential value for large-scale HPV screening.