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毛细管电泳、变性色谱、免疫吸附、DNA测序方法等已广泛用于DNA碱基错配、氧化缺失、链断裂等变化的检测,但有时如进行药物筛选时,只需定性地检测DNA是否变化或变化程度.本文采用褶合光谱法定性地检测紫外线致DNA变化程度,将突变后DNA的褶合光谱与未变异前DNA自身标准比较,并以差谱值δ量化地表示突变程度.当褶合光谱δ高达11.48%时,才能从二阶导数光谱上发现差异,表明方法的灵敏度远远高于前者.加入DMSO后,溶液在254nm照射时,δ升高,表现为DNA变异诱导剂;溶液在365 nm照射时,δ降低,表现为DNA变异保护剂.褶合光谱法快速、简便、灵敏、经济,可以作为检测DNA突变、筛选抗突变药物的一种新型方法.“,”Electrophoresis, chromatography, immunoassay, sequencing and other time consuming approaches have been developed to determine DNA base mismatching, oxidative lesion or strand breaks. Sometimes,however, only qualitative information is enough to decide whether mutation has happened to DNA and its extent.Convolution spectrometry (CS), a new technique to discover ultrafine difference on ultraviolet (UV) absorption of different substances, is originally employed to find out any subtle mutation of DNA induced by UV radiation. Mutative DNA is compared with ego criteria based on the spectra of the former DNA, any difference is quantitatively expressed by dispersion (δ). Visible changes cannot be observed on second -derivative spectra until the mutation gets δup to 11.48%. Dimethyl sulfoxide is an intensifier of UV 254 nm induced DNA mutation and protector at 365 nm,which is simply confirmed by increasing and decreasing δ. Every convolution procedure takes less than 1 min. Convolution spectrometry provides a fast, simple, sensitive and inexpensive alternative to determine DNA mutation, and to screen anti-mutational medicines.