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剥去杏(Prunus armeniaca)果肉,砸开果核并小心取出种子放入消过毒的三角瓶中。接种前用70％酒精浸泡1min,再用0.1％升汞浸泡15min,最后用无菌水冲洗3次,随后在超净工作台上去掉种皮,将胚接种在附加不同浓度6-BA的改良MS培养基(1/2MS大量元素+1/2MS微量元素+1％蔗糖+0.8％琼脂)中。接种后不经低温处理直接放入培养室(26±2℃,3000lx,每天光照16h)培养,10d后调查幼苗生长情况,结果如下:
Peel Prunus armeniaca pulp, break open the kernel and carefully remove the seeds into a sterilized flask. Before inoculation, soak in 70% alcohol for 1min, then soak in 0.1% mercuric chloride for 15min, and finally rinse with sterile water for 3 times. Then remove the seed coat on the clean bench and inoculate the embryos into modified 6-BA with different concentrations MS medium (1 / 2MS large elements + 1 / 2MS trace elements + 1% sucrose + 0.8% agar). After inoculation without temperature treatment directly into the training room (26 ± 2 ℃, 3000lx, light 16h) cultured, 10d after the investigation of seedling growth, the results are as follows: