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应用定向克隆方法，将日本血吸虫虫卵ｃＤＮＡ片段重组入噬菌体载体λｇｔ１１Ｓｆｉ－Ｎｏｔ的ＥｃｏＲⅠ和ＮｏｔⅠ双酶切位点之间。所构建的基因表达文库的容量为１．０７×１０７重组子。经含有ＩＰＴＧ及Ｘ－Ｇａｌ的颜色选择平皿测定，初步提示重组效率达１００％。
The cDNA fragment of Schistosoma japonicum eggs was recombined into EcoRⅠ and NotⅠ restriction sites of λgt11Sfi-Not of phage vector by directional cloning method. The constructed gene expression library has a capacity of 1.07 × 107 recombinants. The color selection plate containing IPTG and X-Gal assay, preliminary suggested that the recombination efficiency of 100%.