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目的 :采用PCR法检测胃癌患者人乳头病毒 (HPV) 16、18型DNA序列 ,探讨HPV类型与人胃腺癌的关系 ,为今后进行胃癌基因治疗及预防提供理论及实验依据。方法 :选择 2 9例胃癌 ,12例慢性胃炎 ,2例不典型增生胃炎患者 ,取癌变组织、癌前病变组织及正常胃组织进行PCR法检测HPV16、18型DNA。结果 :胃癌组织中HPV 16型阳性率 31% ,HPV 18型阳性率 2 4% ;胃腺癌组织中HPV16型阳性率 2 6 % ,HPV 18型阳性率 2 1.7% ;慢性胃炎伴不典型增生HPV16、18型阳性率各为 5 0 % ;而对照组慢性胃炎则未检出。结论 ;HPV16、18型与人胃腺癌有密切关系 ,HPV16、18型感染后可能作为胃癌的一种始动因子 ,开始作用于癌前病变 ,其DNA整合于异型增生细胞内 ,参与内源激活各种致癌因素
Objective: To detect the DNA sequence of human papillomavirus (HPV) type 16 and 18 in patients with gastric cancer by PCR and to explore the relationship between HPV types and human gastric adenocarcinoma and to provide theoretical and experimental evidences for gene therapy and prevention of gastric cancer in the future. Methods: Twenty-nine cases of gastric cancer, 12 cases of chronic gastritis and 2 cases of atypical gastritis were selected. The cancerous tissues, precancerous lesions and normal gastric tissues were collected for detection of HPV16,18 DNA by PCR. Results: The positive rate of HPV type 16 in gastric cancer tissues was 31%, the positive rate of HPV type 18 was 24%. The positive rate of HPV16 in gastric adenocarcinoma was 26% and the positive rate of HPV 18 was 27.7%. The chronic gastritis with atypical hyperplasia HPV16 , 18 positive rate of 50% each; while the control group chronic gastritis was not detected. Conclusion HPV16 and HPV18 are closely related to human gastric adenocarcinoma. HPV16 and HPV18 may play an important role in the carcinogenesis of precancerous lesions and their DNA is integrated in dysplastic cells and participate in endogenous activation A variety of carcinogenic factors