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目的:通过引发技术,探讨增强夏枯草种子耐盐性的有效途径。方法:以不同浓度的NaCl溶液模拟盐分胁迫;引发处理种子置于0.8%NaCl盐胁迫下发芽。结果:随着盐胁迫浓度增加,夏枯草种子发芽率、发芽指数、活力指数下降;经过15%~35%PEG,100~500 mg.L-1GA3,0.4%~2.0%KNO3-KH2PO4引发处理,3个居群夏枯草种子在盐胁迫下种子发芽指数以及活力指数显著提高;NaCl引发,夏枯草种子在胁迫条件下发芽率降低。结论:适量浓度聚乙二醇(PEG),赤霉素(GA3),KNO3-KH2PO4引发,可以增强夏枯草种子萌发阶段耐盐能力。
Objective: To explore the effective ways to enhance the salt tolerance of Prunella vulgaris seeds by inducing technology. Methods: Salt stress was simulated by different concentrations of NaCl solution. The seeds of the induced seedlings were germinated under salt stress of 0.8% NaCl. Results: The germination rate, germination index and vigor index of Prunella vulgaris decreased with the increase of salt stress concentration. After 15 ~ 35% PEG, 100 ~ 500 mg.L-1GA3, 0.4% ~ 2.0% KNO3-KH2PO4, The seed germination index and vigor index of the three populations of Prunella vulgaris seeds under salt stress increased significantly. Under NaCl stress, the germination rate of Prunella vulgaris seeds under stress decreased. Conclusion: The appropriate concentration of polyethylene glycol (PEG), gibberellin (GA3) and KNO3-KH2PO4 can enhance the salt tolerance of Prunella vulgaris seed germination stage.