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目的:建立满山红中金丝桃苷的高效液相色谱分析法。方法:采用C18柱,流动相为甲醇:水:0.1%磷酸=35:20:45,检测波长为260nm。结果:精密度和稳定性良好,金丝桃苷在0.2ug～1.0ug范围内呈现良好的线性关系。结论:本方法简便、专属、重现性好,可有效控制满山红药材的质量。
Objective: To establish a HPLC method for the determination of hyperoside in mangosteen. Methods: C18 column was used. The mobile phase was methanol: water: 0.1% phosphoric acid = 35:20:45 and the detection wavelength was 260 nm. Results: The precision and stability were good. Hyperoside showed a good linearity in the range of 0.2ug ~ 1.0ug. Conclusion: The method is simple, specific, reproducible, and can effectively control the quality of Manshanhong herbs.