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放线菌ＳＩＰＩ－９７６４的发酵液经溶媒萃取、硅胶柱层析和结晶等，分离出２个异黄酮类化合物，命名为ＳＩＰＩ－９７６４－Ⅰ和－Ⅱ。通过对其理化性质和ＵＶ、ＩＲ、ＭＳ、ＮＭＲ图谱的分析，确定化合物ＳＩＰＩ－９７６４－Ⅰ和－Ⅱ分别为４′，５，７－Ｔｒｉｈｙｄｏｘｙｉｓｏｆｌａｖｏｎｅ和４′，７－Ｄｉｈｙｄｒｏｘｙｉｓｏｆｌａｖｏｎｅ，与已知的金雀异黄素（ｇｅｎｉｓｔｅｉｎ）和黄豆苷元（Ｄａｉｄｚｅｉｎ）相一致。两个化合物显示出一定的Ｎａ＋／Ｋ＋ＡＴＰａｓｅ抑制活性和抗虫活性，其在１ｍｇ／ｍｌ的浓度对线虫的击倒率分别为７３％和６４％。
Actinomycete SIPI-9764 fermentation broth by solvent extraction, silica gel column chromatography and crystallization, isolated two isoflavones compounds, named SIPI-9764-Ⅰ and-Ⅱ. The compounds SIPI-9764-I and -II were determined to be 4 ’, 5,7-Trihydoxyisoflavone and 4’, 7-Dihydroxyisoflavone, respectively, by their physicochemical properties and UV, IR, MS and NMR spectra. Genistein and Daidzein are consistent. The two compounds showed certain Na + / K + ATPase inhibitory activity and insecticidal activity, and the knockdown rates of nematodes at the concentration of 1 mg / ml were 73% and 64% respectively.