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利用２９０Ｇｙ６０Ｃｏ—γ射线处理细胞质雄性不育系Ⅱ—３２Ａ和协青早Ａ，获得了育性恢复突变体，突变频率分别为８×１０－３和５×１０－３。从Ⅱ—３２Ａ中所获得的育性恢复突变体，在农艺性状上与Ⅱ—３２Ｂ基本相同。已稳定的Ⅱ－３２Ａ育性恢复突变体Ⅱ－３２Ｒ，与Ⅱ－３２Ａ和珍汕９７Ａ测交，杂种结实率在７０％以上。对测交后代的遗传分析表明，Ⅱ—３２Ｒ对珍汕９７Ａ和Ⅱ—３２Ａ的育性恢复均涉及２对恢复基因、Ⅱ—３２Ｒ与珍汕９７Ａ和Ⅱ—３２Ａ的杂种Ｆ２代在结实率分布上存在差异，Ⅱ—３２Ａ／Ｔ—１８和Ⅱ—３２Ａ／Ｔ—２１具有相同的趋势，但珍汕９７Ａ／Ｔ—１８趋向低结实率端，Ｔ—１８和Ｔ—２１为Ⅱ—３２Ｒ—１和Ⅱ—３２Ｒ—２中选出的结实率较高的两株系。诱导不育系产生育性恢复突变体是获得细胞质雄性不育系恢源的一种有效方法。
Fertility restoration mutants were obtained using 290Gy60Co-γray treatment of cytoplasmic male sterile line Ⅱ-32A and Xieqingzao A, with mutation frequencies of 8 × 10-3 and 5 × 10-3, respectively. Fertility restoration mutants obtained from Ⅱ-32A were almost identical to Ⅱ-32B in agronomic traits. The stable Ⅱ-32A fertility restorer mutant Ⅱ-32R was detected with Ⅱ-32A and Zhenshan 97A, and the seed-setting rate of hybrid was above 70%. The genetic analysis of the tested crosses showed that the fertility restoration of both Zhenshan 97A and Ⅱ-32A by Ⅱ-32R involved two F2 pairs of restorer genes, the F2 generation of F2-32R and Zhenshan 97A and Ⅱ-32A, T-18 and T-21 were Ⅱ-32R-T and T-18 and T-21 were the lowest in Zhenshan 97A / T-18, 1 and Ⅱ-32R-2 selected higher seed setting rate of the two strains. Induction of sterile lines to produce fertility restorers is an effective way to obtain cytoplasmic male sterile lines.