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目的：探讨壶腹癌中抑癌基因Ｐ１６ 蛋白与增殖细胞核抗原（ＰＣＮＡ）表达的相关性及其与肿瘤临床病理特征的关系。方法：采用免疫组织化学ＡＢＣ法检测４５例壶腹癌及１１ 例癌旁正常胰腺组织中Ｐ１６ 蛋白及ＰＣＮＡ的表达，并与临床病理学进行对比分析。结果：Ｐ１６蛋白在壶腹癌和癌旁正常胰腺组织中的阳性表达率有显著性差异（Ｐ＜ ０．０５）；Ｐ１６ 蛋白表达阳性率在肿瘤分化程度低、有胰头侵犯、淋巴结转移、临床分期高者显著降低；ＰＣＮＡ 蛋白阳性表达在有淋巴结转移者明显升高，与是否侵犯胰头及肿瘤大小无关；Ｐ１６ 与ＰＣＮＡ 的表达呈明显的负相关。结论：Ｐ１６与ＰＣＮＡ的表达异常与壶腹癌的发生、发展密切相关，联合检测Ｐ１６ 蛋白和ＰＣＮＡ可作为判断肿瘤恶性程度的参考指标
Objective: To investigate the relationship between the expression of tumor suppressor gene P16 protein and proliferating cell nuclear antigen (PCNA) in ampullary carcinoma and its relationship with clinicopathological features of the tumor. Methods: Immunohistochemical ABC method was used to detect the expression of P16 protein and PCNA in 45 cases of ampullary carcinoma and 11 cases of adjacent normal pancreatic tissues. The results were compared with clinical pathology. Results: The positive expression rate of P16 protein in ampullary carcinoma and para-tumor normal pancreas was significantly different (P<0.05); the positive rate of P16 protein expression was low in tumor differentiation, pancreatic head invasion, lymph node metastasis, The clinical stage was significantly lower; the positive expression of PCNA protein was significantly increased in those with lymph node metastasis, which was not related to the invasion of pancreatic head and tumor size. The expression of P16 was significantly negatively correlated with the expression of PCNA. Conclusion: The abnormal expression of P16 and PCNA is closely related to the occurrence and development of ampullary carcinoma. Combined detection of P16 protein and PCNA can be used as a reference index to judge the malignancy of the tumor.