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近来,人们发现在单层培养的人羊膜细胞分泌的前列腺素烷类(Prostanoids)中以 PGE_2 量最大。实验证明人羊膜细胞单层培养时,其 PG 生物合成及代谢特征和人的羊膜组织一样。本文旨在建议应用培养基的羊膜细胞作为研究羊膜组织调节PGE_2 生物合成的极好模型。取正常妊娠剖腹产妇女的胎盘,移去羊膜后,培养单层羊膜细胞。用特异放免法测定前列腺素烷类的含量,用 Lowry 法测定羊膜细胞的蛋白含量。每次实验前要更换培养基并定为零点。实验开始,分别把各细胞平皿的培养基取出供测定下列 PG 用。如 PGE_2,PGF_(2α)、6-酮-PGF_(1α)、促凝血素(TxB_2)、
Recently, it was found that monolayer cultured human amniotic cells secreted prostaglandins (Prostanoids) in the largest amount of PGE_2. Experiments show that human amniotic cell monolayer culture, its PG biosynthesis and metabolic characteristics and human amniotic tissue. This article aims to recommend the use of culture medium amnion cells as an excellent model for the study of amnion tissue to regulate PGE 2 biosynthesis. Take normal pregnancy caesarean section of women’s placenta, remove the amniotic membrane, monolayer cultured amniotic cells. The contents of prostaglandin alkaloids were determined by radioimmunoassay, and the protein content of amniotic cells was determined by Lowry method. Before each experiment to change the medium and set as zero. At the beginning of the experiment, the culture medium of each cell plate was taken out for determination of the following PG. Such as PGE_2, PGF_ (2α), 6-keto-PGF_ (1α), thrombin (TxB_2)