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目的研究2009年湖州市流感的病原甲型H1N1分离株A/Huzhou/09(H1)的HA和NA基因特征。方法采用MDCK细胞对患者咽拭子和漱口液标本进行病毒分离,逆转录-聚合酶链反应(RT-PCR)扩增病毒HA和NA基因并进行序列测定,用DNAstar和MegAlign等软件分析处理。结果湖州市甲型H1N1分离株的HA和NA基因为1706个核苷酸和1401个核苷酸,与其他地区分离株核苷酸同源性在99.5%～100.0%和99.7%～100.0%。进化树分析显示,湖州市甲型H1N1分离株(A/Huzhou/09)的HA和NA基因与其他地区分离株变化不大,几乎完全一致。结论甲型H1N1分离株的变异特征及亲缘进化,分析对流感的流行病学研究具有重要意义。
Objective To study the HA and NA gene characteristics of the influenza A H1N1 isolate A / Huzhou / 09 (H1) in Huzhou City in 2009. Methods The virus was isolated from throat swabs and mouthwash samples by MDCK cells. The HA and NA genes were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced. DNAstar and MegAlign software . Results The HA and NA genes of the H1N1 isolates in Huzhou City were 1706 nucleotides and 1401 nucleotides in nucleotide sequence. The nucleotide homologies of isolates from other regions were 99.5% -100.0% and 99.7% -100.0%. Phylogenetic tree analysis showed that the HA and NA genes of Huzhou A / H1N1 isolate (A / Huzhou / 09) did not change much and almost the same with other isolates. Conclusion The variation characteristics and phylogenetic analysis of influenza A (H1N1) isolates are of great significance to the epidemiological study of influenza.