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目的研究葫芦素E(cucurbitacin E,CuE)体外对人肝癌BEL7402和HepG2细胞的增殖抑制作用及机制。方法MTT法检测不同浓度CuE体外对BEL7402和HepG2细胞的增殖抑制作用;流式细胞仪检测CuE对细胞周期分布的影响;Western blot法检测CuE对细胞周期调节蛋白cy-clinB1、Cdc25C、Cdk1及p21表达的影响。结果CuE作用72 h可剂量依赖性地抑制BEL7402和HepG2细胞增殖;CuE(100 nmo.lL-1)作用12、24 h后可使BEL7402和HepG2细胞周期阻滞于G2/M期,并可下调Cdk1蛋白的表达,上调p21蛋白的表达。结论CuE体外可抑制人肝癌BEL7402和HepG2细胞的增殖,其增殖抑制作用与诱导G2/M周期阻滞有关。
Objective To study the inhibitory effect and mechanism of cucurbitacin E (CuE) on the proliferation of human hepatocellular carcinoma BEL7402 and HepG2 cells in vitro. Methods MTT assay was used to detect the inhibitory effect of different concentrations of CuE on the proliferation of BEL7402 and HepG2 cells in vitro. Flow cytometry was used to detect the effect of CuE on cell cycle distribution. Western blot was used to detect the effects of CuE on cell cycle regulatory proteins cy-clinB1, Cdc25C, Cdk1 and p21 The impact of expression. Results CuE treatment inhibited the proliferation of BEL7402 and HepG2 cells in a dose-dependent manner for 72 h. After treated with CuE (100 nmo.lL-1) for 12 and 24 h, the cell cycle of BEL7402 and HepG2 cells were arrested in G2 / M phase and down-regulated Cdk1 protein expression, up-regulation of p21 protein expression. Conclusion CuE can inhibit the proliferation of human hepatocellular carcinoma BEL7402 and HepG2 cells in vitro, and its inhibitory effect on proliferation is related to the induction of G2 / M cycle arrest.