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以东方百合‘索邦’鳞片为试材,研究鳞片扦插诱导小鳞茎形成的过程及其鳞片内部糖和蛋白质的分布特点。经石蜡切片PAS和氧化汞—溴酚蓝组织化学染色观察发现:(1)小鳞茎起源于近轴基部8～14层薄壁细胞,为内起源;形态发生过程依次经过了启动、生长锥形成、叶原基形成和小鳞茎形成;小鳞茎的形成与分生细胞团所在位置有关。(2)小鳞茎发生过程中多糖主要以颗粒状存在于分裂旺盛的分生组织和维管组织周围,多糖含量高时有利于诱导形成维管组织;蛋白质主要集中于细胞核周围;多糖和蛋白质在分生组织及其周围细胞之间存在着浓度梯度,由顶端分生组织细胞到周围的薄壁细胞中,蛋白质含量由高到低,多糖含量则由低到高。
The oriental lily ’Sorbonne’ scale was used as test material to study the process of scale-forming process and the distribution of sugar and protein in scales. The paraffin sections of PAS and mercury oxide - bromophenol blue histochemical staining showed that: (1) Bulblets originated in the plexiform cells at the base of 8 to 14 plexiform layers, which were internal origin; morphogenesis took place through the start, growth cone formation , Leaf primordium formation and bulb formation; the formation of bulblets and meristematic cell clusters are located. (2) In the process of bulblets, polysaccharides mainly exist in granular form around the meristematic meristematic tissue and vascular tissue. When the content of polysaccharides is high, it is conducive to the formation of vascular tissue; the protein mainly concentrates in the nucleus; the content of polysaccharides and protein in There is a concentration gradient between the meristem and its surrounding cells, from apical meristematic cells to surrounding parenchyma cells, from high to low protein content, low to high polysaccharide content.